仓储作者

[中文专利] 一种β-葡萄糖醛酸苷酶及其重组表达载体、工程菌、发酵剂和量产甘草次酸的方法

发明人：吕波，陈林浩，赖俊杰，李春

申请人：北京理工大学

申请号： 202410627081.1

申请日期： 2024.05.20

摘要：本发明一种β‑葡萄糖醛酸苷酶及其重组表达载体、工程菌、发酵剂和量产甘草次酸的方法属于酶工程和微生物技术领域。所述β‑葡萄糖醛酸苷酶选自：β‑葡萄糖醛酸苷酶AcGUS、AcGUS1、AcGUS2、AcGUS3、AcGUS3突变体；AcGUS为GenBank登录号为AEK69352.1的氨基酸序列的第1 ...

[中文专利] 三个UDP-糖基转移酶及其在合成齐墩果酸糖苷化合物中的应用

发明人：冯旭东，孙秋艳，孟亮旗，刘护，李春

申请人：北京理工大学

申请号： 202410433715.X

申请日期： 2024.04.11

摘要：发明“三个UDP‑糖基转移酶及其在合成齐墩果酸糖苷化合物中的应用”属于生物工程与技术领域。本发明提供一种UDP‑糖基转移酶，涉及三个来源于油橄榄的UDP‑糖基转移酶Oe440、Oe139、Oe535，实现在齐墩果酸C28‑COOH特异性修饰一分子糖基生成28‑氧‑葡萄糖‑齐墩果酸；提供了一株工程化酿 ...

[中文专利] 一种生产石竹烯的基因工程菌株及其构建方法

发明人：李春，张亚鹏，吕波，秦磊

申请人：清华大学，北京理工大学

申请号： 202410644736.6

申请日期： 2024.05.23

摘要：本发明涉及基因工程领域，具体涉及生产石竹烯的基因工程菌株及其构建方法。该基因工程菌株的出发菌株为酿酒酵母；在基因工程菌株的基因组中，第一基因的内源启动子被替换为第一启动子，其中第一基因选自由ERG9、ERG1、ERG7和ERG11组成的组；第一启动子独立地选自由以下组成的组：P_ERG1

[外文期刊] Efficient production of 22(R)-hydroxycholesterol via combination optimization of Saccharomyces cerevisiae SCIE EI

作者： Pang, Yaru¹;Cheng, Xu¹;Ban, Yali²;Li, Yue¹;Lv, Bo¹;Li, Chun^1,3 （¹Beijing Inst Technol, Sch Chem & Chem Engn, Key Lab Med Mol Sci & Pharmaceut Engn, Minist Ind & Informat Technol,Inst Biochem Engn, Beijing 100081, Peoples R China.;²Beijing Inst Technol, Sch Life Sci, Beijing, Peoples R China.;³Tsinghua Univ, Dept Chem Engn, Key Lab Ind Biocatalysis, Minist Educ, Beijing 100084, Peoples R China.）

出处： BIOTECHNOLOGY JOURNAL 2024 Vol.19 No.7

关键词： BIOSYNTHESIS; ACID; CHOLESTEROL; EXPRESSION; YEAST

摘要： 22(R)-hydroxycholesterol (22(R)-HCHO) is a crucial precursor of steroids biosynthesis with various biological functions. However, the production of 22 ...

[外文期刊] Self-controlled in silico gene knockdown strategies to enhance the sustainable production of heterologous terpenoid by Saccharomyces cerevisiae SCIE

作者： Zhang, Na¹;Li, Xiaohan¹;Zhou, Qiang¹;Zhang, Ying¹;Lv, Bo¹;Hu, Bing¹;Li, Chun^1,2 （¹Inst Biochem Engn, Beijing Inst Technol, Sch Chem & Chem Engn, Key Lab Med Mol Sci & Pharmaceut Engn,Minist Ind &, Beijing 102401, Peoples R China.;²Tsinghua Univ, Key Lab Ind Biocatalysis, Minist Educ, Dept Chem Engn, Beijing 100084, Peoples R China.）

出处： METABOLIC ENGINEERING 2024 Vol.83

关键词： KNOCKOUT STRATEGIES; FRAMEWORK; DESIGN; MODEL; ACID

摘要： Microbial bioengineering is a growing field for producing plant natural products (PNPs) in recent decades, using heterologous metabolic pathways in ho ...

[外文期刊] Low temperature promotes the production and efflux of terpenoids in yeast SCIE

作者： Qin, Lei^1,2,3;Ma, Dongshi⁴;Lin, Guangyuan^1,2,3;Sun, Wentao^1,2,3;Li, Chun^1,2,3,4 （¹Tsinghua Univ, Ctr Synthet & Syst Biol, Beijing, Peoples R China.;²Tsinghua Univ, Dept Chem Engn, Beijing, Peoples R China.;³Tsinghua Univ, Key Lab Ind Biocatalysis, Minist Educ, Beijing, Peoples R China.;⁴Beijing Inst Technol, Sch Chem & Chem Engn, Key Lab Med Mol Sci & Pharmaceut Engn, Minist Ind & Informat Technol,Inst Biochem Engn, Beijing, Peoples R China.）

出处： BIORESOURCE TECHNOLOGY 2024 Vol.395

关键词： LIPID-METABOLISM GENES; SACCHAROMYCES-CEREVISIAE; FERMENTATION; BIOSYNTHESIS; RESISTANCE; PROTEINS; GROWTH

摘要： Altering the fermentation environment provides an effective approach to optimizing the production efficiency of microbial cell factories globally. Her ...

[外文期刊] Modulation signal bispectrum analysis of motor current signals for online monitoring of turning conditions

作者： Zou, Zhexiang^{1, 2}; Li, Chun^{1, 2}; Shen, Guoji³; Li, Dongqin¹; Gu, Fengshou²; Ball, Andrew David²; （¹School of Industrial Automation, Beijing Institute of Technology, Zhuhai, China;²Centre for Efficiency and Performance Engineering, University of Huddersfield, Huddersfield, United Kingdom;³College of Intelligence Science and Technology, National University of Defense Technology, Guangdong, Zhuhai, China）

出处： Measurement and Control (United Kingdom) 2024 Vol.57 No.5 P540-550

摘要： Maintaining exceptional product quality and boosting processing efficiency requires precise evaluation of various aspects of the turning process, incl ...

[外文期刊] Efficient glycyrrhetinic acid biomanufacturing through protein engineering and dual-GUS combination strategy with novel β-glucuronidase from Aspergillus calidoustus CLH-22 SCIE EI

作者： Chen, Linhao¹;Lai, Junjie¹;Luo, Yan¹;Shu, Tao¹;Lv, Bo¹;Li, Chun^1,2 （¹Beijing Inst Technol, Inst Biochem Engn, Sch Chem & Chem Engn, Key Lab Med Mol Sci & Pharmaceut Engn,Minist Ind &, Beijing 102488, Peoples R China.;²Tsinghua Univ, Dept Chem Engn, Key Lab Ind Biocatalysis, Minist Educ, Beijing 100084, Peoples R China.）

出处： BIORESOURCE TECHNOLOGY 2024 Vol.413

关键词： GLYCYRRHIZIC ACID; EXTRACTION; MECHANISM

摘要： Glycyrrhetinic acid (GA) is a high-value pentacyclic triterpenoid with broad applications. However, the industrial production of GA is hindered by low ...

[外文期刊] Gearbox Lubricant Condition Monitoring Based on Motor Current Signals from Closed-Loop Control Drive EI

作者： Zou, Zhexiang¹; Li, Chun¹; Li, Dongqin¹; Shen, Guoji²; Gu, Fengshou³; Ball, Andrew D.³ （¹School of Industrial Automation, Beijing Institute of Technology, Zhuhai, China;²College of Intelligence Science and Technology, National University of Defense Technology, Changsha, China;³Centre for Efficiency and Performance Engineering, University of Huddersfield, Huddersfield, UK）

出处： IEEE Transactions on Instrumentation and Measurement 2024 P1-1

[外文期刊] Engineering the Substrate Specificity of UDP-Glycosyltransferases for Synthesizing Triterpenoid Glycosides with a Linear Trisaccharide as Aided by Ancestral Sequence Reconstruction

作者： Xing Jian¹;Qiuyan Sun¹;Wentao Xu¹;Haobo Qu¹;Xudong Feng¹;Chun Li²; （¹ Beijing Institute of Technology, Department of Biochemical Engineering, No 5, Zhongguancun South Street, 100081, Beijing, CHINA. ² Tsinghua University, Department of Chemical Engineering, 100084, Beijing, CHINA.）

出处： Angewandte Chemie (International ed. in English) 2024 Pe202409867

关键词： UDP-glycosyltransferase;biocatalysis;protein engineering;reconstructed ancestral enzyme;triterpenoid 2"-O-glycosylation.

摘要： Triterpenoids have wide applications in the pharmaceutical and agricultural industries. The glycosylation of triterpenoids catalyzed by UDP-glycosyltr ...