作者：杨成佳¹;，成旭¹;，胡冰¹;，吕波¹;，李春^1,2; （¹北京理工大学化学与化工学院；²清华大学化学工程系）

出处：生物加工过程 2023

关键词：三萜化合物；P450酶；催化机制；催化特异性；CPR适配

摘要：植物三萜化合物是一类具有6个C₅异戊二烯单元的高附加值天然化合物，具有抗炎、护肝、抗肿瘤、抗氧化和降血压等重要药理活性。在三萜化合物生物合成过程中，细胞色素P450酶通过引入羟基、羧基、羰基以及环氧基等官能团，为丰富三萜结构的多样性起到了重要的作用。然而，目前P450酶底物催化 ...

发明人：冯旭东，郭芳，李春，刘护

申请日期：2022.06.14

摘要：本发明公开了一种重组载体、转化体及其在五环三萜类化合物糖基化修饰中的应用，具体地，本发明的毕赤酵母重组载体和/或其转化体和/或重组载体组合物和/或转化体组合物和/或细胞表面共展示糖基转移酶和蔗糖合酶的重组毕赤酵母，用于五环三萜类化合物的糖基化修饰，具有重复利用率高，催化效率高，反应条件温和，高效，绿 ...

发明人：邹江猛，杨珊珊，韦金鹏，黄健菁，韩智，李春

申请日期：2022.05.18

摘要：本实用新型公开了一种动力合成机构及水中垃圾清理设备，该动力合成机构包括第一增速组件、第二增速组件和速度合成组件；第一增速组件和第二增速组件用于对转动驱动力进行增速；速度合成组件包括第一传动轴、第二传动轴、第一圆锥齿轮、第二圆锥齿轮、第三圆锥齿轮、第四圆锥齿轮和输出件；第一增速组件的输出端通过第一传动 ...

发明人：李春，韩智，黄健菁，韦金鹏，杨珊珊，邹江猛

申请日期：2022.05.18

摘要：本实用新型公开了一种水中油污收集机构及水中清洁设备，该水中油污收集机构包括本体、漂浮件、活动件、传动装置和转动件；转动件转动设置于本体上，转动件上设置有吸油层；漂浮件沿自身长度方向设置有固定件，活动件的一端活动设置于固定件上，且活动件的另一端通过传动装置与转动件连接，漂浮件在水中漂浮时，活动件相对固 ...

发明人：李春，林煜彬，黄石青，莫伟强，邓荣峰，王红军，朱泓博

申请日期：2022.01.12

摘要：本实用新型属于切削刀具技术领域，公开了一种切削刀柄检测系统，包括传感器、控制器、电源装置以及套设于切削刀柄外的第一套件和第二套件，可以独立于切削刀柄，不需要对切削刀柄本身的结构进行改造。通过将第一套件和第二套件分别固定于切削刀柄上，传感器安装于第一套件上，控制器和电源装置分别安装于第二套件上，控制器 ...

作者：Fang Guo;Mingzhu Liu;Hu Liu;Chun Li;Xudong Feng （¹ Key Laboratory of Medical Molecule Science and Pharmaceutical Engineering, Ministry of Industry and Information Technology, Institute of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing100081, China. ² Key Lab for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing100084, China.）

出处：ACS synthetic biology 2023

关键词：enzyme cascades;glycosylation;natural products;substrate channeling;surface display.

摘要：Yeast surface display is an appealing technique for constructing multienzyme cascades. This technique is commonly achieved using a scaffold for the or ...

作者：Ting An;Xudong Feng;Chun Li （¹ Key Laboratory of Medical Molecule Science and Pharmaceutical Engineering, Ministry of Industry and Information Technology, Institute of Biochemical Engineering, Department of Chemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China. ² Department of Chemical Engineering, Key Lab for Industrial Biocatalysis, Ministry of Education, Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China.）

出处：Journal of agricultural and food chemistry 2023

关键词：aromatic prenyltransferases;biocatalysis;biomanufacturing;microbial cell factories;prenylated aromatic natural products.

摘要：Prenylated aromatic natural products (PANPs) have received much attention due to their biomedical benefits for human health. The prenylation of aromat ...

作者：Xia Liu;Lei Qin;Jie Yu;Wentao Sun;Jianhong Xu;Chun Li （¹ Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, Institute of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, China. ² Department of Chemical Engineering, Tsinghua University, Beijing, China; Key Lab for Industrial Biocatalysis, Ministry of Education, Tsinghua University, Beijing, China; Center for Synthetic and Systems Biology, Tsinghua University, Beijing, China. ³ Department of Chemical Engineering, Tsinghua University, Beijing, China. ⁴ Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, Institute of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, China; Department of Chemical Engineering, Tsinghua University, Beijing, China; Key Lab for Industrial Biocatalysis, Ministry of Education, Tsinghua University, Beijing, China; Center for Synthetic and Systems Biology, Tsinghua University, Beijing, China. Electronic address: lichun@tsinghua.edu.cn.）

出处：Biosensors & bioelectronics 0

关键词：Cell compartment;Fluorescent protein;Redox biosensor system (RBS);Redox state;Saccharomyces cerevisiae.

摘要：During industrial fermentation, microbial cell factories are usually confronted with environmental or metabolic stresses, leading to the imbalance of ...

作者：Qibin Wang;Dingyuan Lu;Hu Liu;Chun Li （¹ Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, Institute of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 10081, PR China. ² Key Laboratory for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing 10084, PR China. ³ Center for Synthetic & Systems Biology, Tsinghua University, Beijing 10084, PR China.）

出处：Journal of agricultural and food chemistry 0

关键词：glycyrrhizin hydrolysis;oligomeric structures;subunit dissociation;tetramerization;β-glucuronidases.

摘要：In this study, structural analysis was employed to identify three hotspot residues that contribute most to the tetramer formation of a glycoside hydro ...

作者：Xing Jian¹;Chun Li^{1   2   3};Xudong Feng¹; （¹ Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, Institute of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, China. ² Key Lab for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing, China. ³ Center for Synthetic & Systems Biology, Tsinghua University, Beijing, China.）

出处：Critical reviews in biotechnology 2022

关键词：Glycoside hydrolases;engineered transglycosylases;product polymerization degree;substrate specificity;transglycosylation activity.

摘要：Glycosides are widely used in many fields due to their favorable biological activity. The traditional plant extractions and chemical methods for glyco ...